Metabolomics of biological systems
Metabolomics is a relatively new field of science that deals with the analysis of the metabolome, the collection of all low molecular weight substances (usually less than 1,500 Da) in and outside living organisms. This analysis is challenging due to the high temporal dynamics of the metabolome and the highly variable concentrations of metabolites. However, knowledge of the metabolome and its changes in the context of different phenomena (pathological states of the organism, altered diet, different plant species) can often provide crucial insights into understanding these phenomena.
Our group analyses the metabolome of mainly biological samples (body fluids, tissues) and plants and describes changes in the metabolome in connection with many phenomena. For metabolome analysis, we use various mass spectrometric techniques, often in combination with chromatographic methods, which allow us to identify individual metabolites associated with the changes studied in organisms.
Publications
- Uttl L., Bechynska K., Ehlers M., Kadlece V., Navratilova K., Dzuman Z., Fauhl-Hassek C., Hajslova J.: Critical assessment of chemometric models employed for varietal authentication of wine based on UHPLC-HRMS data. Food Control (2023) 143: 109336. (doi: 10.1016/j.foodcont.2022.109336)
- Fiserova I., Trinh M.D., Elkalaf M., Vacek L., Heide M., Martinkova S., Bechynska K., Kosek V., Hajslova J., Fiser O., Tousek P., Polak J.: Isoprenaline modified the lipidomic profile and reduced β-oxidation in HL-1 cardiomyocytes: In vitro model of takotsubo syndrome. Frontiers in Cardiovascular Medicine (2022) 9:917989. (doi: 10.3389/fcvm.2022.917989)
- Garcia C.J., Kosek V., Beltran D., Tomas-Barberan F.A., Hajslova J.: Production of New Microbially Conjugated Bile Acids by Human Gut Microbiota. Biomolecules (2022) 12(5): 687. (doi: 10.3390/biom12050687)
- Kosek V., Hajsl M., Bechynska K., Kucerka O., Suttnar J., Hlavackova A., Hajslova J., Maly M.: Long-Term Effects on the Lipidome of Acute Coronary Syndrome Patients. Metabolites (2022) 12(2): 124. (doi: 10.3390/metabo12020124)
- Smid V., Dvorak K., Sedivy P., Kosek V., Lenicek M., Dezortova M., Hajslova J., Hajek M., Vitek L., Bechynska K., Bruha R.: Effect of Omega-3 Polyunsaturated Fatty Acids on Lipid Metabolism in Patients With Metabolic Syndrome and NAFLD. Hepatology Communications (2022) 6(6): 1336-1349. (doi: 10.1002/hep4.1906)
- Vacek L., Dvorak A., Bechynska K., Kosek V., Elkalaf M., Trinh M.D., Fiserova I., Pospisilova K., Slovakova L., Vitek L., Hajslova J., Polak J.: Hypoxia Induces Saturated Fatty Acids Accumulation and Reduces Unsaturated Fatty Acids Independently of Reverse Tricarboxylic Acid Cycle in L6 Myotubes. Frontiers in Endocrinology (2022). 13: 663625. (doi: 10.3389/fendo.2022.663625)
- Bechynska K., Kosek V., Fenclova M., Muchova L., Smid V., Suk J., Chalupsky K., Sticova E., Hurkova K., Hajslova J., Vitek L., Stranska M.: The Effect of Mycotoxins and Silymarin on Liver Lipidome of Mice with Non-Alcoholic Fatty Liver Disease. Biomolecules (2021) 11: 1723. (doi: 10.3390/biom11111723)
- Bechynska K., Kosek V., Zlechovcova M., Peukertova P., Hajlsova J.: Cannabis Metabolomic Data Processing: Challenges to be Addressed. LCGC (2021) Special Issues 34(s10): 11-15. (on-line)
- Birse N., Chevallier O., Hrbek V., Kosek V., Hajslova J., Elliot Ch.: Ambient mass spectrometry as a tool to determine poultry production system history: A comparison of rapid evaporative ionisation mass spectrometry (REIMS) and direct analysis in real time (DART) ambient mass spectrometry platforms. Food Control (2021). 123: 107740 (doi: 10.1016/j.foodcont.2020.107740)
- Maly M., Hajsl M., Bechynska K., Kucerka O., Sramek M., Suttnar J., Hlavackova A., Hajslova J., Kosek V.: Lipidomic analysis to assess oxidative stress in acute coronary syndrome and acute stroke patients. Metabolites (2021) 11(7): 412. (doi: 10.3390/metabo11070412)
- Bechynska K., Daskova N., Vrzackova N., Harant K., Heczkova M., Podzimkova K., Bratova M., Dankova H., Berkova Z., Kosek V., Zelenka J., Hajslova J., Sedlacek R., Suttnar J., Hlavackova A., Bartonova L., Cahova M.: The effect of omega-3 polyunsaturated fatty acids on the liver lipidome, proteome and bile acid profile: parenteral versus enteral administration. Scientific Reports (2020) 9: 19097. (doi: 10.1038/s41598-019-54225-8)
- Kosek V., Heczkova M., Novak F., Meisnerova E., Novakova O., Zelenka J., Bechynska K., Vrzacova N., Suttnar J., Hlavackova A., Dankova H., Bratova M., Daskova N., Malinska H., Oliyarnyk O., Wohl P., Bastova H., Cahova M.: The ω-3 polyunsaturated fatty acids and oxidative stress in long-term parenteral nutrition dependent adult patients: Functional lipidomics approach. Nutrients (2020) 12(8) 2351. (doi: 10.3390/nu12082351)
Projecsts
AZV
- NU22-04-00389: Vliv metabolomu aterosklerotického plátu v karotidě na jeho embolizační potenciál (2022 - 2025)
- NU21-01-00259: Nové inhibitory lipolýzy v léčbě poruch metabolismu lipidů a glukózy při syndromu obstrukční spánkové apnoe (2021 – 2024)
- NV18-08-00149: Kritické zhodnocení lipidomu u pacientů s akutním koronárním syndromem a cévní mozkovou příhodou ve vztahu ke stupni oxidačního stresu (2018 – 2021)