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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2015
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DEPARTMENT OF FOOD ANALYSIS AND NUTRITION is a part of the Faculty of Food and Biochemical Technology. The research projects are mainly focused on food and feed quality, authenticity & safety related issues; investigations concerned with the impact of environmental conditions on the human food chain are also included. Special attention is paid to studies concerned with food components interactions / breakdown which may result in changes of nutritional value and/or sensory properties. In the recent years, interdisciplinary ‘omics’-based research aimed at the assessment of in vivo / in vitro effects induced by biologically active compounds, both beneficial and toxic, has been initiated.

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Our Department contributes to teaching of following Study Programmes:

 

Bachelor Study Programme

Chemistry and Technology, specialization Food Chemistry and Technology

Our teachers participate in learning of following subjects of this programme:

2nd year

Food Chemistry (SIS or EMIL)

Sensory Analysis (SIS or EMIL)

3rd year

Analysis of Food and Natural Products: Laboratory (SIS or EMIL)

Human Nutrition and Nutritional Policy (SIS or EMIL)

Food and Natural Products Analysis (SIS or EMIL)

 

Master Study Programmes

Biotechnology and Food Science 

Our teachers participate in learning of following subjects of this programme:

1st year

Reaction Mechanisms in Food Chemistry (SIS or EMIL)

Special Food Analysis (SIS or EMIL)

Chemical Food Safety (SIS or EMIL)

Analysis of Bioactive Compounds (SIS or EMIL)

Control of Food Raw Materials and Commodities: Laboratory (SIS or EMIL)

2nd year

Isolation and Separation Methods (SIS or EMIL)

 

Doctoral Study Programme

Food and Natural Products

List of available PhD topics here 

 

 

 

 

 

 

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Expert services and/or collaboration within common research projects are offered in the following areas:

A. FOOD/FEED CHEMICAL SAFETY, HUMAN EXPOSURE, AND FORENSIC ISSUES

Development of advanced methods for analysis of multiple contaminants and their metabolites in food, feed, biological and environmental matrices; investigation of preventive measures and mitigation strategies:

  • Mycotoxins and natural plant toxins
  • Pesticide / veterinary drugs residues
  • POPs and other industrial contaminants
  • Packaging contaminants / migrants
  • Processing contaminants, antinutritive substances
  • Nanoparticles
  • ´Emerging´ contaminants

B. FOOD QUALITY ATTRIBUTES AND AUTHENTICATION; BIOPROSPECTION

Implementation of novel approaches for characterization and classification of:

  • Metabolome / foodome: fingerprinting and/or profiling methods employing high resolution MS (ambient, LC, GC); advanced data processing; specific markers´ identification
  • Biologically active compounds: non-target screening for ‘unknowns’ in plants / microorganisms; identification based on cutting-edge mass spectrometric techniques; assessment of chemical cocktails in combination with bioactivity testing
  • Flavours / odours: combination of instrumental and sensory analysis; assessment based on the combination of comprehensive GC and olfactometry

 

TRAINING AND STUDY STAYS are offered to PhD students / postdoc young researchers interested in the above areas.

The UCT Prague closely cooperates with EU Reference Laboratories (EURLs) network in the field of QA/QC strategies, interlaboratory validation studies, reference materials and traceability of chemical measurements.

ACCREDITED TESTING AND METROLOGICAL LABORATORY’ of the Department of Food Analysis and Nutrition performs according to the International standard ISO/IEC 17025:2005; accreditation was provided by the Czech Accreditation Institute, signatory of the ILAC-MRA. For an external QC, the laboratory regularly participates in the proficiency testing (FAPAS, EU PT, IRMM, IAEA, etc.).

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DATA


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  • Nicolet iS50 FT-IR Spectrometer (Thermo Fisher Scientific, USA)
Description

Fourier transform infrared spectrometer Nicolet TM iS50 (FTIR)
This instrumentation represents Fourier transform infrared spectrometer (FT-IR) with a built-in diamond ATR crystal for measurements using an attenuated total reflection technique. Measurements can be performed in UV-VIS, IR, NIR, and FAR area (without the need of blowing through the device). The instrument is also able to record Raman spectra without any replacement of the system components. Infrared spectroscopy is widely used in both qualitative and quantitative analysis. At the Department of Food Analysis and Nutrition UCT Prague, this instrument is mostly used for determination of the acid value or authentication of various foodstuffs and food supplements (such as oil, butter, margarine, spices, honey, lecithin, oyster mushroom, milk thistle).
Publications:
• Vytejčková S., Gavlasová D., Hajšlová J., Poustka J.: Fourier transform infrared spectroscopy coupled to chemometric tools applied for the authentication of flours made of cereals and pseudo cereals. 7th International Symposium on Recent Advances in Food Analysis, November 3-6, 2015, Prague, Czech Republic
• Vytejčková S., Pohořelá B., Pánek J., Hajšlová J., Poustka J.: Recognition of vegetable oils adulteration and oxidative changes in thermally stressed fats and oils using Fourier transform infrared spectroscopy. 7th International Symposium on Recent Advances in Food Analysis, November 3-6, 2015, Prague, Czech Republic
• Pekárková V., Vassilkova A., Hajšlová J., Poustka J.: Potential of Fourier transform infrared spectroscopy for joint supplements authentication. 7th International Symposium on Recent Advances in Food Analysis, November 3-6, 2015, Prague, Czech Republic
• Poustka J.: Application of mid infrared spectroscopy for food and food supplements authentication. 9th International Symposium on Recent Advances in Food Analysis, November 5-8, 2019, Prague, Czech Republic

  • Spectrophotometer Cary 60 UV-Vis (Agilent Technologies, USA)

  • Agilent 1200 Series Rapid Resolution LC system  with DAD and FLD detectors (Agilent Technologies, USA) 
Description

The liquid chromatography system connected with a diode array detector and a fluorescence detector is mainly used for the analysis of polycyclic aromatic hydrocarbons, carotenoids, amino acids and vitamins (groups B, C and E) in a wide range of foods (fruits, vegetables, teas, cereals, food supplements, hemp products…) and beverages.

Publications:
• Pavelkova P., Krmela A., Schulzova V.: Determination of carotenoids in flowers and food supplements by HPLC-DAD. Acta Chimica Slovaca (2020) 13(1). (doi: 10.2478/acs-2020-0002)
• Bhave A., Schulzova V., Mrnka L., Hajslova J.: Influence of Harvest Date and Postharvest Treatment on Carotenoid and Flavonoid Composition in French Marigold Flowers. Journal of Agricultural and Food Chemistry (2020) 68(30): 7880–7889. (doi: 10.1021/acs.jafc.0c02042)
• Novotny C., Schulzova V., Krmela A., Hajslova J., Svobodova K., Koudela M.: Ascorbic Acid and Glucosinolate Levels in New Czech Cabbage Cultivars: Effect of Production System and Fungal Infection. Molecules (2018) 23(8):1855 (doi: 10.3390/molecules23081855)

  • Acquity UPLC with Evaporative Light Scattering (ELS) detector (Waters, USA)

  • FCPC® A Kromaton (Rousselet Centrifugation)
Description

Fast centrifugal partitioning chromatography FCPC® A Kromaton coupled with peripheral system puriFlash®4250 (Interchim)
This instrumentation consists of a fast centrifugal chromatography (FCPC® A Kromaton) with a rotor volume of 1000 ml and a peripheral system puriFlash®4250 (Interchim), which includes a pump, injection valve, 50 ml injection loop, PDA detector (200 – 600 nm) and fraction collector. The separation is based on the different affinity of the analytes to the two immiscible solvent mixtures, which represent the stationary and mobile phase. The system is suitable for the separation and isolation of various bioactive compounds, such as phenolics, flavonoids, lignans, alkaloids, and many others.

Main benefits of the FCPC® A Kromaton system

• Large amounts of sample mass can be loaded (up to 50 ml)
• No extraction and purification of target molecules needed prior FCPC
• Suitable even for low-concentrated bioactive compounds • Applicable for a broad spectrum of compounds
At the Department of Food Analysis and Nutrition UCT Prague, this instrument is mainly used for the separation and isolation of bioactive compounds from Cannabis sativa L. extracts.

  • Halogen Moisture Analyzer HG63 (Mettler Toledo) 
Description

The halogen moisture analyzer is suitable for routine determination of moisture content. It is fast (results obtained within minutes) and efficient, and offers very good repeatability even if a small amount of sample is available. The fast speed and good repeatability of the measurement are achieved by a circular halogen lamp and a gold-plated reflector. The results are obtained with resolution of up to 10 ppm MC. The halogen moisture analyzer is mainly used for the determination of the dry matter in plant material.

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UCT Prague
Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373

Czech Post certified digital mail code: sp4j9ch

Copyright: UCT Prague 2015
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre.
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