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Technická 5
166 28 Prague 6 – Dejvice
IČO: 60461373 / VAT: CZ60461373
Czech Post certified digital mail code: sp4j9ch
Copyright: UCT Prague 2015
Information provided by the Department of International Relations and the Department of R&D. Technical support by the Computing Centre.
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[obsah] => DEPARTMENT OF FOOD ANALYSIS AND NUTRITION is a part of the Faculty of Food and Biochemical Technology. The research projects are mainly focused on food and feed quality, authenticity & safety related issues; investigations concerned with the impact of environmental conditions on the human food chain are also included. Special attention is paid to studies concerned with food components interactions / breakdown which may result in changes of nutritional value and/or sensory properties. In the recent years, interdisciplinary ‘omics’-based research aimed at the assessment of in vivo / in vitro effects induced by biologically active compounds, both beneficial and toxic, has been initiated.
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Our Department contributes to teaching of following Study Programmes:
Bachelor Study Programme
Chemistry and Technology, specialization Food Chemistry and Technology
Our teachers participate in learning of following subjects of this programme:
2nd year
Sensory Analysis (SIS or EMIL)
3rd year
Analysis of Food and Natural Products: Laboratory (SIS or EMIL)
Human Nutrition and Nutritional Policy (SIS or EMIL)
Food and Natural Products Analysis (SIS or EMIL)
Master Study Programmes
Biotechnology and Food Science
Our teachers participate in learning of following subjects of this programme:
1st year
Reaction Mechanisms in Food Chemistry (SIS or EMIL)
Special Food Analysis (SIS or EMIL)
Chemical Food Safety (SIS or EMIL)
Analysis of Bioactive Compounds (SIS or EMIL)
Control of Food Raw Materials and Commodities: Laboratory (SIS or EMIL)
2nd year
Isolation and Separation Methods (SIS or EMIL)
Doctoral Study Programme
Food and Natural Products
List of available PhD topics here
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[obsah] => Expert services and/or collaboration within common research projects are offered in the following areas:
A. FOOD/FEED CHEMICAL SAFETY, HUMAN EXPOSURE, AND FORENSIC ISSUES
Development of advanced methods for analysis of multiple contaminants and their metabolites in food, feed, biological and environmental matrices; investigation of preventive measures and mitigation strategies:
- Mycotoxins and natural plant toxins
- Pesticide / veterinary drugs residues
- POPs and other industrial contaminants
- Packaging contaminants / migrants
- Processing contaminants, antinutritive substances
- Nanoparticles
- ´Emerging´ contaminants
B. FOOD QUALITY ATTRIBUTES AND AUTHENTICATION; BIOPROSPECTION
Implementation of novel approaches for characterization and classification of:
- Metabolome / foodome: fingerprinting and/or profiling methods employing high resolution MS (ambient, LC, GC); advanced data processing; specific markers´ identification
- Biologically active compounds: non-target screening for ‘unknowns’ in plants / microorganisms; identification based on cutting-edge mass spectrometric techniques; assessment of chemical cocktails in combination with bioactivity testing
- Flavours / odours: combination of instrumental and sensory analysis; assessment based on the combination of comprehensive GC and olfactometry
TRAINING AND STUDY STAYS are offered to PhD students / postdoc young researchers interested in the above areas.
The UCT Prague closely cooperates with EU Reference Laboratories (EURLs) network in the field of QA/QC strategies, interlaboratory validation studies, reference materials and traceability of chemical measurements.
‘ACCREDITED TESTING AND METROLOGICAL LABORATORY’ of the Department of Food Analysis and Nutrition performs according to the International standard ISO/IEC 17025:2005; accreditation was provided by the Czech Accreditation Institute, signatory of the ILAC-MRA. For an external QC, the laboratory regularly participates in the proficiency testing (FAPAS, EU PT, IRMM, IAEA, etc.).
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GC-MS/MS Agilent 7000B Triple Quadrupole
Gas chromatography coupled to tandem mass spectrometry (triple quadrupole) equipped with electron impact ionization technique is employed for the analysis of pesticide residues in a wide range of foodstuffs (e.g. fruit, vegetable, tea, cereals). This instrumentation allows to measure more than 300 pesticide residues in less than 30 minutes at ultra-trace concentrations in one run. This system is also equipped with a JetClean function for self-cleaning ion source with hydrogen.
Publications
Drabova L., Alvarez-Rivera G., Suchanova M., Schusterova D., Pulkrabova J., Tomaniova M., Kocourek V., Chevallier O., Elliot Ch., Hajslova J.: Food fraud in oregano: pesticide residues as adulteration markers. Food Chemistry (2019) 276:726-734. (doi:10.1016/j.foodchem.2018.09.143)
Cajka T., Sandy C., Bachanova V., Drabova L., Kalachova K., Pulkrabova J., Hajslova J.: Streamlining sample preparation and gas chromatography-tandem mass spectrometry analysis of multiple pesticide residues in tea. Anal. Chim. Acta (2012) 743:51–60. (doi: 10.1016/j.aca.2012.06.051)
GC-MS/MS Agilent 7000B Triple Quadrupole
Gas chromatography coupled to tandem mass spectrometry (triple quadrupole) is particularly used for the analysis of environmental contaminants (e.g. polychlorinated biphenyls, flame retardants, organochlorine pesticides). Applications allow multi-detection analysis in a wide range of environmental (e.g. soil or atmosphere), food (e.g. meat, fat, fruit or vegetable) and biological (e.g. human breast milk or human blood serum) samples. This second triplequad system is also possible to operate in chemical ionization suitable particularly for the analysis of halogenated compounds and it is equipped with fully automated device for solid phase microextraction (CTC Pal) for the analysis of volatile compounds.
Publications
Polachova A., Gramblicka T., Parizek O., Sram R.J., Stupak M., Hajslova J., Pulkrabova J.: Estimation of human exposure to polycyclic aromatic hydrocarbons (PAHs) based on the dietary and outdoor atmospheric monitoring in the Czech Republic. Environmental Research (2020) 182: 108977. (doi: 10.1016/j.envres.2019.108977)
Logerova H., Tuma P., Stupak M., Pulkrabova J., Dlouhy P.: Evaluation of the burdening on the Czech population by brominated flame retardants. International Journal of Environmental Research and Public Health (2019) 16(21) 4105. (doi: 10.3390/ijerph16214105 )
Pulkrabova J., Cerny J., Szakova J., Svarcova A., Gramblicka T., Hajslova J., Balik J., Tlustos P.: Is the long-term application of sewage sludge turning soil into a sink for organic pollutants?: evidence from field studies in the Czech Republic. Journal of Soils and Sediments (2019) 19: 2445-2458. (doi:10.1007/s11368-019-02265-y)
Svarcova A., Lankova D., Gramblicka T., Stupak M., Hajslova J., Pulkrabova J.: Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies. Science of The Total Environment (2019) 667: 701-709. (doi: 10.1016/j.scitotenv.2019.02.336)
Szakova J., Pulkrabova J., Cerny J., Mercl F., Svarcova A., Gramblicka T., Najmanova J., Tlustos P., Balik J.: Selected persistent organic pollutants (POPs) in the rhizosphere of sewage sludge-treated soil: Implications for the biodegradability of POPs. Archives of Agronomy and Soil Science (2019) 65(7): 994-1009. (doi:10.1080/03650340.2018.1543945)
Pulkrabova J., Stupak M., Svarcova A., Rossner P., Rossnerova A., Ambroz A., Sram R., Hajslova J.: Relationship between atmospheric pollution in the residential area and concentrations of polycyclic aromatic hydrocarbons (PAHs) in human breast milk. Science of the Total Environment (2016) 562: 640-647. (doi: 10.1016/j.scitotenv.2016.04.013)
Kalachova K., Cajka T., Sandy C., Hajslova J., Pulkrabova J.: High throughput sample preparation in combination with gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-MS/MS): a smart procedure for (ultra)trace analysis of brominated flame retardants in fish. Talanta (2013) 105:109–116.(doi: 10.1016/j.talanta.2012.11.073)
Kalachova K., Pulkrabova J., Cajka T., Drabova L., Stupak M., Hajslova J.: Gas chromatography–triple quadrupole tandem mass spectrometry: A powerful tool for the (ultra)trace analysis of multi-class environmental contaminants in fish and fish feed. Anal. Bioanal. Chem., (2013) 405: 7803–7815 (doi: 10.1007/s00216-013-7000-4)
GC-MS/MS Agilent 7000C Triple Quadrupole
Gas chromatography coupled to tandem mass spectrometry (triple quadrupole) is an alternative technique to a system Agilent 7000B for the analysis of environmental contaminants (e.g. polychlorinated biphenyls, flame retardants, organochlorine pesticides). Applications allow multi-detection analysis in a wide range of environmental (e.g. soil or atmosphere), food (e.g. meat, fat, fruit or vegetable) and biological (e.g. human breast milk or human blood serum) samples. By Agilent 7000C system we can achieved lower limits of quantification for target analytes compared to the system Agilent 7000B. This system is also equipped with a JetClean function for self-cleaning ion source with hydrogen.
GC-MS/MS Agilent 7010 Triple Quadrupole
The last generation of gas chromatography coupled to tandem mass spectrometry (triple quadrupole) is employed for the analysis of pesticide residues in a wide range of foodstuffs (e.g. fruit, vegetable, tea, cereals) and environmental samples. This instrument allows to measure more than 300 pesticide pesticides in less than 30 minutes in one run. Its high-efficiency electron impact ion source produces at least 20 times as many ions as the previous generation, improving the efficiency of the entire workflow.
GC-HRMS Pegasus HRT (LECO)
Gas chromatography coupled to high resolution mass spectrometry (time of flight analyzer) enable resolution of 50,000 FWHM and mass accuracies less than 1 ppm is used for target and non-target analysis of volatile and semi-volatile compounds. This technique is equipped with a fully automated device for solid phase microextraction (Gerstel). GC-HRMS Pegasus HRT is employed for non-target analysis of volatiles in variable samples, for a routine analysis of furans or dithiocarbamates and for a practical training of students.
Publications
Gracka A., Raczyk M., Hradecky J., Hajslova J., Jeziorski S., Karlovits G., Michalak B., Bąk N. and Jeleń H.: Volatile compounds and other indicators of quality for cold-pressed rapeseed oils obtained from peeled, whole, flaked, and roasted seeds. European Journal of Lipid Science Technology (2017) 119 (10). (doi:10.1002/ejlt.201600328)
Hradecky J., Kludska E., Belkova B., Wagner M., Hajslova, J.: Ohmic heating: A promising technology to reduce furan formation in sterilized vegetable and vegetable/meat baby foods. Innovative Food Science and Emerging Technologies (2017) 43: 1-6. (doi: 10.1016/j.ifset.2017.07.018)
GC-MS Agilent 5973 Single Quadrupole
Gas chromatography coupled to mass spectrometry (single quadrupole) is particularly used in student laboratory courses. In the courses, students learn detail functions of this instrumentation and try some GC-MS application (e.g. the determination of volatile compounds in spices or the alcoholic beverages quality determination).
Publications
Stupak M, Kocourek V., Kolouchova I., Hajslova J.: Rapid approach for the determination of alcoholic strength and overall quality check of various spirit drinks and wines using GC–MS. Food Control (2017) 80: 307-313 (doi: 10.1016/j.foodcont.2017.05.008)
GCxGC-MS Pegasus® 4D
Gas chromatography enabling two dimensional separation coupled to mass spectrometry (time of flight analyzer) is particularly used for the determination of volatile and semi-volatiles compounds in extremely complex matrixes (e.g. mineral oils). This technique has fully automated device for solid phase microextraction (Gerstel). Other applications employing GCxGC-MS Pegasus® 4D are the analysis of environmental contaminants (e.g. polychlorinated biphenyls, flame retardants or organochlorine pesticides) and terpenoic compounds in cannabis and cannabis products.
Publications
Gracka A., Raczyk M., Hradecky J., Hajslova J., Jeziorski S., Karlovits G., Michalak B., Bąk N. and Jeleń H.: Volatile compounds and other indicators of quality for cold-pressed rapeseed oils obtained from peeled, whole, flaked, and roasted seeds. European Journal of Lipid Science Technology (2017) 119 (10) . (doi:10.1002/ejlt.201600328)
Drabova L., Pulkrabova J., Kalachova K., Tomaniova M., Kocourek V., Hajslova J.: Polycyclic aromatic hydrocarbons (PAHs) and halogenated persistent organic pollutants (POPs) in canned fish and seafood products: smoked versus non-smoked products. Food Addit. Contam. A (2013) 30:5152–527. (doi: 10.1080/19440049.2012.755645)
Drabova L., Tomaniova M., Kalachova K., Kocourek V., Hajslova J., Pulkrabova J.: Application of solid phase extraction and two-dimensional gas chromatography coupled with time-of-flight mass spectrometry for fast analysis of polycyclic aromatic hydrocarbons in vegetable oils. Food Control (2013) 33: 489–497. (doi: 10.1016/j.foodcont.2013.03.018)
Drabova L., Pulkrabova J., Kalachova K., Tomaniova M., Kocourek V., Hajslova J.: Rapid determination of polycyclic aromatic hydrocarbons (PAHs) in tea using Two-Dimensional Gas Chromatography coupled with Time of Flight Mass Spectrometry. Talanta (2012) 100:207–216. (doi:10.1016/j.talanta.2012.07.081)
Kalachova K., Pulkrabova J., Cajka T., Drabova L., Hajslova J.: Implementation of comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for the simultaneous determination of halogenated contaminants and polycyclic aromatic hydrocarbons in fish. Anal. Bioanal. Chem. (2012) 403:2813–2824. (doi: 10.1007/s00216-012-6095-3)
Drábová L., Pulkrabová J., Kalachová K., Hradecký J., Suchanová M., Tomaniová M., Kocourek V., Hajšlová J.: Novel approaches to determination of PAHs and halogenated POPs in canned fish. Czech J. Food Sci. (2011) 29:498–507
Kalachova K., Pulkrabova J., Drabova L., Cajka T., Kocourek V., Hajslova J.: Simplified and rapid determination of polychlorinated biphenyls, polybrominated diphenyl ethers, and polycyclic aromatic hydrocarbons in fish and shrimps integrated into a single method. Anal. Chim. Acta (2011) 707:84–91. (doi: 10.1016/j.aca.2011.09.016)
GC-HRMS Agilent 7200b
Agilent 7200b is a system consists of gas chromatography and high resolution tandem mass spectrometry (quadrupole-time of flight analyzer). This instrumentation is mostly applied for non-target analysis (e.g. determination of Scotch whisky authenticity) as well as for target analysis (e.g. chlorinated paraffins in foodstuffs and biological samples, pesticide residues in foodstuffs, terpenoic compounds in cannabis and cannabis products and determination of spirits quality). This instrument is also equipped with fully automated device for solid phase microextraction and chemical (positive/negative) ionization. Agilent 7200b is also used in student laboratory courses.
Publications
Stupak M., Goodall I., Tomaniova M., Pulkrabova J., Hajslova J.: A novel approach to assess the quality and authenticity of Scotch Whisky based on gas chromatography coupled to high resolution mass spectrometry. Analytica Chimica Acta (2018) 1042:60-70. (doi: 10.1016/j.aca.2018.09.017)
Stupak M, Kocourek V., Kolouchova I., Hajslova J.: Rapid approach for the determination of alcoholic strength and overall quality check of various spirit drinks and wines using GC–MS. Food Control (2017) 80: 307-313 (doi: 10.1016/j.foodcont.2017.05.008)
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